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human primary dental pulp cells dpc  (ATCC)


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    ATCC human primary dental pulp cells dpc
    M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and <t>DPC</t> via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human <t>primary</t> <t>fibroblasts;</t> DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.
    Human Primary Dental Pulp Cells Dpc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+dental+pulp+cells+dpc/pmc10585237-36-6-28?v=ATCC
    Average 99 stars, based on 8458 article reviews
    human primary dental pulp cells dpc - by Bioz Stars, 2026-07
    99/100 stars

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    1) Product Images from "Mycoplasma hyorhinis infection promotes TNF-α signaling and SMAC mimetic-mediated apoptosis in human prostate cancer"

    Article Title: Mycoplasma hyorhinis infection promotes TNF-α signaling and SMAC mimetic-mediated apoptosis in human prostate cancer

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2023.e20655

    M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and DPC via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human primary fibroblasts; DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.
    Figure Legend Snippet: M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and DPC via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human primary fibroblasts; DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Infection, In Vitro, Western Blot



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    human primary dental pulp cells dpc  (ATCC)
    99
    ATCC human primary dental pulp cells dpc
    M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and <t>DPC</t> via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human <t>primary</t> <t>fibroblasts;</t> DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.
    Human Primary Dental Pulp Cells Dpc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+primary+dental+pulp+cells+dpc/pmc10585237-36-6-28?v=ATCC
    Average 99 stars, based on 1 article reviews
    human primary dental pulp cells dpc - by Bioz Stars, 2026-07
    99/100 stars
    		  Buy from Supplier

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    M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and DPC via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human primary fibroblasts; DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.

    Journal: Heliyon

    Article Title: Mycoplasma hyorhinis infection promotes TNF-α signaling and SMAC mimetic-mediated apoptosis in human prostate cancer

    doi: 10.1016/j.heliyon.2023.e20655

    Figure Lengend Snippet: M. hyorhinis promotes TNF-α secretion from PCa cells. (A), relative TNF-α secretion levels in M. hyorhinis-contaminated PCa cells (PC3-cM and C4–2B-cM) and mycoplasma-free cells; multiple cancer cells (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63), HDMEC, HF, and DPC via ELISA. (B), experimental design. The parental PCa cells (PC3–P and C4–2B–P) were infected with M. hyorhinis (3 × 107 CFU, 5 MOI) for 2 passages and then passaged twice a week for 6 weeks without further infection (PC3-M and C4–2B-M). For PC3-MF and C4–2B-MF cells, 4 weeks after infection, M. hyorhinis was eliminated in cell cultures for 3 weeks. Seven weeks after infection, in vitro assays and analyses were performed using these PCa cells. (C), relative TNF-α secretion levels in the parental (PC3–P and C4–2B–P) and M. hyorhinis-infected (PC3-M and C4–2B-M) PCa cells. (D), relative TNF-α secretion levels in PC3-M and C4–2B-M and previously infected PCa cells after elimination of M. hyorhinis (PC3-MF and C4–2B-MF). M. hyrorhinis infection was confirmed by Western blotting using specific anti-M. hyorhinis (P70 surface antigen) antibody. βactin was used as a loading control. See full images in Supplementary Figure S4. All results represent mean ± SD values from triplicate assays, and the experiments were repeated three times. **p < 0.0001. HDMEC, human dermal microvascular endothelial cells; HF, human primary fibroblasts; DPC, human primary dental pulp cells; CFU, colony-forming units; MOI, multiplicity of infection.

    Article Snippet: Human primary fibroblasts (HF) [ ], human primary dental pulp cells (DPC) [ ], and human cancer cell lines (PC3, C4–2B, DU145, LNCaP, MDA-MB-231, MCF-7, MCF10A, HeLa, MG-63; American Type Culture Collection (ATCC), Manassas, VA) were grown in Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Carsbad, CA) supplemented with 10 % FBS and 1 % penicillin/streptomycin.

    Techniques: Enzyme-linked Immunosorbent Assay, Infection, In Vitro, Western Blot